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KMID : 0370219780220040233
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Yakhak Hoeji
1978 Volume.22 No. 4 p.233 ~ p.237
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Studies on the Chain Anomaly Determination of Human Globins
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노일협/Ro IH
최정숙/Choi JS
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Abstract
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We studied on the separation of alpha- and beta-chain globins, the conditions of paper electrophoresis and mobility of globins in paper electrophoresis to determine their chain anomaly. Acidic-acetone mothod of Wilson and Smith was found to be more effecient for separating alpha-and beta-chain globins than trichloroacetic acid method of Hayashi. The conditions of paper electrophoresis were current of 20v/cm and 0.6mA/cm for three hours at room temperature. In paper electroporesis, the mobility of a-chain globin towards the cathode was greater than that of @-chain globin. The results showed that chain anomaly of alpha- and beta-chain globins can be determined by the method of paper electrophoresis.
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